This core provides comprehensive support for the cytogenetic analysis of cells from human and research animal sources. A wide range of chromosome analysis techniques are available; ranging from basic G-banded karyotyping, to 24-color Spectral Karyotyping (SKY), as well as Fluorescence in situ Hybridization (FISH) mapping of cloned DNAs.
Services include:
Karyotype characterization of new cell lines
Confirmation of cell line identity or stability
Karyotype screening of ES cells prior to transgenesis
Mapping of transgene integration sites
Chromosome or locus copy number enumeration
Publication-quality illustrations
Cytogenetic techniques available:
Short-term cell culture
Metaphase chromosome preparation
G-banded karyotyping
FISH techniques available:
DNA labeling for custom probe generation (for FISH projects)
DNA amplification by DOP-PCR
Chromosome painting
FISH mapping (on chromosomes, interphase nuclei or DNA fibers)
Tissue FISH (paraffin or frozen sections)
Immuno-FISH (DNA FISH combined with fluorescent immunostaining)
Spectral karyotyping (SKY)
Q-FISH (measurement of telomere lengths)
The core manager will discuss individual projects with investigators and provide advice on the appropriate type of materials required and the techniques for achieving the most effective outcome. Requests for additional chromosomal staining techniques not specifically listed above can usually be accommodated.
